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RNeasy Plus Mini and Micro Kits OverviewAdding the Plus to your RNA extractionProcedureDiscoverthe PlusEfficientRNA IsolationSuperiorDNA RemovalProvenTechnologyAutomated onQIAcubeOrderingInformationSample to Insight

RNeasy Plus Mini & Micro KitsRNeasy Plus Mini ProcedureOverviewProcedureProcedure Enrichment of mRNA; depletion of small RNAs* upplementary protocol for purification of small SRNAs incl. miRNAs from cultured cellsEfficientRNA Isolation ffective removal of DNA with gDNA eliminator EcolumnsSuperiorDNA Removal Neasy Plus Micro Kit for even the smallest Rsample amountsAutomated onQIAcubeOrderingInformation NA suitable for any downstream application, Reven the most sensitive onesDownloadSupplementaryProtocolGenomic DNATotal RNAgDNA Eliminatorspin column:Remove genomic DNAAdd ethanolRNeasy spin column:bind total RNATotal RNAWashEluteEluted RNA* For isolation of miRNA, please see miRNeasy KitsSample to InsightLyse and homogenize Purify RNA ( 200 bp) in less than 25 minutesDiscoverthe PlusProvenTechnologyCells or tissueTotal time: 25 minutes

RNeasy Plus Mini & Micro KitsOverviewProcedureDiscoverthe PlusEfficientRNA IsolationSuperiorDNA RemovalProvenTechnologyAutomated onQIAcubeOrderingInformationSample to InsightAdding the Plus to RNeasy Kits: Effective gDNA removal with gDNA eliminator columns Phenol-free RNA isolation Faster and simpler than DNase treatment More cost-efficient than DNase I digest Optimal results for any downstream application

RNeasy Plus Mini & Micro KitsOverviewProcedureHigh yields of RNA from cells and easy-to-lyse tissuesCT value40Discoverthe PlusEfficientRNA IsolationSuperiorDNA RemovalProvenTechnologyAutomated onQIAcubeOrderingInformationSample to Insight363228242016105RNeasy Plus104103Cell number102Supplier AVRNA isolation from different amounts of Jurkat cells using the RNeasy Plus Mini Kit or a kit from supplier AV. qRT-PCR for β-actin demonstrates greater RNA yields with theRNeasy Plus Kit. The kit from supplier AV detected no transcript in RNA purified from 102 cells.

RNeasy Plus Mini & Micro KitsOverviewProcedureSuperior DNA removal without the need for DNase IDNA (ng/µl)30Discoverthe Plus252015EfficientRNA IsolationSuperiorDNA Removal1050LiverRNeasy PlusProvenTechnologyAutomated onQIAcubeOrderingInformationSample to InsightKidneySupplier TLungSupplier AIVRNA was purified from various mouse tissues (10 mg) using the RNeasy Plus Mini Kit or kits from other suppliers. Real-time PCR assays for c-jun indicate amount of DNAcontamination in the purified RNA

RNeasy Plus Mini & Micro KitsOverviewProcedureMore than 10,000 publications* cite RNeasy PlusPublications3000Discoverthe Plus2300250019502000EfficientRNA IsolationSuperiorDNA 201520162017Automated onQIAcubeOrderingInformation* Google Scholar – Dec 2017Sample to Insight

RNeasy Plus Mini & Micro KitsOverviewProcedureDiscoverthe PlusEfficientRNA IsolationSuperiorDNA RemovalAutomatable onQIAcubeProvenTechnologyAutomated onQIAcubeOrderingInformationSample to Insight NochangeofpurificationchemistrywhenusingtheQIAcube Increased productvity with automated sample extraction Standardized results

RNeasy Plus Mini & Micro KitsOrdering InformationOverviewProductContentsProcedureRNeasy Plus Mini Kit (50)For 50 minipreps: RNeasy Mini Spin Columns, gDNA EliminatorSpin Columns, Collection Tubes, RNase-Free Water and Buffers74134Discoverthe PlusRNeasy Plus Mini Kit (250)For 250 minipreps: RNeasy Mini Spin Columns, gDNA EliminatorSpin Columns, Collection Tubes, RNase-Free Water and Buffers74136EfficientRNA IsolationRNeasy Plus Micro Kit (50)For 50 micropreps: RNeasy MinElute Spin Columns, gDNA EliminatorSpin Columns, Collection Tubes, Carrier RNA, RNase-Free Waterand Buffers74034QIAcubeRobotic workstation for automated purification of nucleic acids orproteins using QIAGEN spin-column kits, 1-year warranty on partsand laborSuperiorDNA RemovalProvenTechnologyCat. no.9001292** QIAcube (110 V) cat. no. 9001292 or QIAcube (230 V) cat. no. 9001293.Automated onQIAcubeOrderingInformationSample to InsightFor up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual.QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Servicesor your local distributor.Trademarks: QIAGEN , Sample to Insight , QIAcube , MinElute , RNeasy (QIAGEN Group). 2018 QIAGEN, all rights reserved.Ordering www.qiagen.com/contact Technical Support support.qiagen.com Website www.qiagen.comVisit Webpage